Theyre Throughout this process, tetrodes were only gripped by a section near the trimmed end that would later be trimmed away. Yang, A. Rys, C. M. McLaughlin, H. F. Sleiman, Templated Ligand Environments for the Selective Incorporation of Different Metals into DNA. WebAlso known as chatter-free tungsten, this material is used for boring bars and grinding tools that dampen noise and vibration while machining. The functional concept will remain the same if the roles are reversed so that the choice can be dictated by application considerations. After the top heater is turned off, the top PCM remains liquid for a short time while the heat diffuses away. It appeared from the design that these formed a coil around the via that might create magnetic fields that would potentially induce currents in the electrodes that could be noisy (making it impossible to evaluate electrode movement using feedback) or even damage the brain tissue. A 3D-printed bracket with a metal bolt was attached to the other end. 114, 168301 (2015). K. Lo, H. F. Sleiman, Synthesis and Molecular Recognition Properties of DNA-Mimetic Conjugated Polymer. I'm milling a bunch of PVC now with HSS and it works fine. The piezo motor is a ceramic block, which could be broken by direct banging to the core MPSA motor. Proc. 98% of products ordered ship from stock and deliver same or next day. eLife's peer-review process is changing. The system combines a single piezo actuator with phase change material on two separate boards to allow multiple wires to be independently controlled via a single actuator, reducing the overall weight of the implant. WebEzineArticles.com allows expert authors in hundreds of niche fields to get massive levels of exposure in exchange for the submission of their quality original articles. Bottom, inter-spike interval histograms and waveforms of the spikes on the four channels for each isolated unit during the full period. How much does the inchworm actuator add in weight to any standard Microdrive? Ma, J., Cahill, D. G. & Miljkovic, N. Condensation induced blistering as a measurement technique for the adhesion energy of nanoscale polymer films. We have addressed this topic in the overall response to Main Concern #3 above and believe that we have provided sufficient explanation and data to alleviate concerns over this issue. 16, 658+ (2017). ISSN 2041-1723 (online). Candle soot as a template for a transparent robust superamphiphobic coating. Conf on Research Trends in Science and Techn, March 2002, Beirut, Lebanon, American Chemical Society Meeting, Pacifichem 2000, Honolulu, Hawai, December 2000. First, the gripper is operated electronically which allows for remote control. Lubricant-infused surfaces for low-surface-tension fluids: promise versus reality. 6, eaax0746 (2020). Jing, B. You accept the use of cookies or other identifiers by closing or dismissing this notice, by scrolling this page, by clicking a link or button or by continuing to browse otherwise. Microsoft pleaded for its deal on the day of the Phase 2 decision last month, but now the gloves are well and truly off. Often used as feet, spacers, and vibration dampers, these bumpers absorb shock to prevent damage to equipment and surfaces. While the design and demonstration of the system are solid and generated significant excitement, the limited demonstration of the robustness of the device in long term many electrode configurations was perhaps incomplete. In a separate experiment, we measured repeatability. Shock wave energy dissipation in catalyst-free poly(dimethylsiloxane) vitrimers. Importantly, the actuation signal to the piezo remains the same. This arrangement accommodates the use of flexible neural probes such as twisted-wire tetrodes and long silicon shanks (Michon et al., 2016) but precludes the use of stiff micropipettes and short silicon shanks which are typically too inflexible to bend along the angular guide channel and through the vertical cannula. WebAlso known as chatter-free tungsten, this material is used for boring bars and grinding tools that dampen noise and vibration while machining. We show that postsynaptic plasticity traces can be induced independently of the presynapse, and that in vivo dynamics of 2 nAChR subunits are changed both in the context of associative and non-associative (familiarity) memory formation, underlying different plasticity rules. Self-cleaning of superhydrophobic surfaces by self-propelled jumping condensate. Soc. Theyre We are Wholesale Suppliers of Premium Cutting tools,Hand tools,Power tools and Accessories. Both approaches have significant disadvantages. New. Publishers note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. An additional safety margin of ~5 for both temperatures was included when analyzing the simulations to conservatively account for potential differences between the model and the fabricated device. (2010-13), Member, College of Reviewers for the Canada Foundation for Innovation, Member, College of Reviewers for the Natural Science and Engineering Council (NSERC) of Canada Special Opportunities Grant (NSERC is the Canadian equivalent to NSF and to the QNRF), Grant Evaluation Panel Member, King Abdullah University of Science and Technology (KAUST), 2010, Member, Advisory Committee for the Selection of a Dean of the Faculty of Engineering, 2012. To learn more, view ourPrivacy Policy. J. For independence, the worst-case thermal scenario is when the central gripper is off but all of the surrounding 15 are on. Therefore, extension or contraction of our piezo actuator itself during motion never extends or contracts the electrode itself. The miniaturization of mechanical, electrical, optical, and other devices has been steadily ongoing for decades, enabled by advances in manufacturing, electronics, and novel mechanical designs. The authors do an excellent job of describing the manufacturing and characterization of their system for arraying grippers to form a multi-electrode microdrive. We thank the reviewer for this comment, which is part of Main Concern #1, which we have addressed above. My understanding from the text and the data in Figure 4 is that these values were tested on only a single tetrode (or two tetrodes for the cross-talk test). During the robotic stepping of the device for electrode translation, described in Figure 2, the heating pulse required for melting of the phase change grippers is extremely short (~10 ms), while the piezo actuators steps (which do not generate heat) are ~1 s long. These components were soldered onto the board before drilling of the microgripper vias. Top, peak amplitude of each spike recorded on two of the four channels of this tetrode during the first and second halves of the period. I do part them off with a HSS blade. Finally, it is possible that enough PCM could be ejected from the grippers as to eventually prevent a solid grip, but PCM can be brought back in by using a particular heating cycle. Rosenberg, B. J. et al. Step duration is marked in gray and corresponds to actuation of the piezo stack. Wisdom, K. M. et al. While the bottom PCM is liquid, the voltage applied to the piezo actuator is set to zero, causing the piezo to contract upward, back to its initial length, as shown in Figure 2A (v). Theyre While the yield of 11 units from the 4 tetrodes in this chronically implanted animal is not particularly high, the stability and cluster quality we observed (according to both visual and quantitative cluster quality metrics spike waveforms, ISI histogram, Isolation Distance, and L-Ratio, as shown in Appendix 1-figure 7 for each cluster) across this period were high, and similar to what is observed with standard, manually adjusted drives. Learn more. Nature 409, 794797 (2001). U.S. Investment in Machine Tools Poised to Reach Remarkable Level, Balanced equations: Toolholder vibration makes for miserable machining, but balancing. OK thanks. Previously, leaving the probes mobile in the bores avoided damage to the probes due to contact with the tweezers and also better controlled the amount of PCM in each microgripper by preventing flow into neighboring bores. Soc. Sequential electronically controlled probe gripping and piezo extension and contraction (described in Figure 2) are used to translate the probe (white dashed arrow points to the probe tip) in either direction (up or down), as shown in F(i)(v) and Video 1. In addition, such manual adjustments are not as precise, because of limitations to manual control and, in screw-based drives, because of backlash. Among her research recognitions are the NSERC Polanyi Award (2021), Cottrell STAR Award (2021), Albus Award (Grifols, 2018), Killam Research Fellowship (2018), Canadian Society of Chemistry R. U. Lemieux Award (2018), Netherlands Scholar Award in Supramolecular Chemistry (2018), Izatt-Christensen Award in Supramolecular Chemistry (2016), Swiss Chemical Society Lectureship (2012), Canadian Institute of Chemistry E. Gordon Young Award (2011), Canadian Society for Chemistry Strem Award (2009), NSERC Discovery Accelerator (2008), McGill Dawson Award (2004-13), Fellow of the Canadian Institute for Advanced Research (2004-12) and Cottrell Scholar (Research Corp., 2002). browsing data or IP addresses) and use cookies or other identifiers, which are necessary for its functioning and required to achieve the purposes illustrated in the cookie policy. We note in particular that in agar the lateral motion is essentially completely eliminated (Figure 4F). Sleiman, Hanadi; Chen, Bingzhi. We isolated 11 single units from the 4 tetrodes. Phase-coupling at slower oscillations in these same frequency bands, in contrast, correlates with longer co-firing latencies and occurs during memory failure. Eng. One possibility is to slightly modify thermally conductive probes by placing a less thermally diffusive sheath around them that would instead be in contact with and delays heat loss from the PCM. We therefore included two guiding PEEK cannulas. Get the most important science stories of the day, free in your inbox. and L.E.P. It is also important to emphasize that during the motion of the electrodes in our device, as diagrammatically shown in Figure 2, the MPSA mechanism does not cause additional axial strain or compression on the electrodes, which would perhaps cause damage if they were. Paxson, A. T. et al. Denissen, W., Winne, J. M. & Du, F. E. Prez, Vitrimers: permanent organic networks with glass-like fluidity. The gripper board designs were simulated before fabrication in order to implement a design with sufficient microgripper thermal independence. Only the largest amplitude spike was taken if multiple threshold crossings occurred within a 1 ms window. describe a system to remotely control the advancement of wires or similarly shaped materials into the brain for in vivo recording. I had a 10" round of grey PVC I had to take quite a lot off of, I just used sharp HHS and ran it fast enough the string was nearly molten, coming off shiny and cooling in the air. From the beginning of the heating pulse to the time of the maximum temperature, the grippers had practically identical temperature rises and did not have a significant effect on each other; the number of grippers on only changed the maximum by ~1C. I'm not asking the authors to rigorously test these different sizes, but rather to give some general guidance based on their experience/knowledge. S. Bazzi, H. F. Sleiman; Adenine-Containing Block Copolymers via Ring-Opening Metathesis Polymerization: Synthesis and Self-Assembly into Rod Morphologies. 6, 581585 (2007). We now provide detailed description, in Figure 2, of the sequence of electronic actuation signals sent to the inchworm microdrive for its three distinct probe translation operations: (a) downward translation step, (b) upward translation step, and (c) no translation step. The samples were attached to a Peltier stage (TP104SC, Instec) oriented vertically using double side copper tape. During this sliding process, the PCM beads were wicked into their bores such that they would grip the probe upon cooling. & Evans, C. M. Catalyst-free dynamic networks for recyclable, self-healing solid polymer electrolytes. advanced. Atmosphere-mediated superhydrophobicity of rationally designed micro/nanostructured surfaces. Finally, as mentioned above, it is our general policy not to ask for significant new experiments. When both pipettes are in air, a single 9.5 m step of the moving pipette caused the stationary one to move momentarily 3.62.1 m on-axis and 23.84.4 m (n=5 steps) off-axis, i.e., laterally (Figure 4F cross-talk in air). We observed an increase in the continuous phaseamplitude relationship selective to the early and late components of the response evoked by lateral temporal stimulation. Nat. WebChoose from over 40,700 shafts in a wide range of sizes. Phys. MPSA Microdrive Tuned Recordings from Chronically Implanted Tetrodes. We therefore left a ~3 mm gap between these and limited the retraction from the maximum depth. We realize that originally we have not properly addressed these heating concerns in our device, but have now added the above points to the revised manuscript. It is important to emphasize that the heating of the brain due to operation of our device is essentially negligible. Nanoengineered materials for liquidvapour phase-change heat transfer. This is critical when precise positioning of the neural electrodes is required for targeted neural recordings. All are reinforced with a base plate for strength. Lett. PubMed To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. What are potential failure points and are they fixable in an implanted animal? WebChoose from our selection of bars, including metal, plastic, and more. Northwestern University, Chemistry Department, Nov. 2-4, 2010. Krutyeva, M. et al. Using evoked component amplitude to measure functional connectivity, we assessed whether the phase of hippocampal theta predicted relatively high versus low connectivity. Initially, the cage, microdrive, and EICB were slightly separated and angled relative to each other such that the holes of the bottom cannula and top board were visible for threading. We would greatly appreciate understanding from the Editor and the Reviewers on this point listed as a secondary concern. To recap, the heat from the grippers does not substantially flow to the brain through the probes. Each tetrode wire was then individually connected to the EICB. Synthesis and Structure of cis-(CO), T. Maxey, H.F. Sleiman and L. McElwee-White; Metathesis and Diaziridination Reactions of (CO), A. Arndtsen, H.F. Sleiman, A.K. Additionally, that small amount of heat energy generated by the heaters within the PCB vias is (by design of the copper layers of the PCB) rapidly dissipated within the large PCB volume, and does not thermally conduct to the brain through the very large thermal resistance of the microscopic recording electrodes. Microsoft pleaded for its deal on the day of the Phase 2 decision last month, but now the gloves are well and truly off. The spectral range for the FTIR Spectrometer used was 8000 cm 1 to 10 cm 1 . Subsequently, the PCM solidifies and the top microgripper re-grips the probe while, importantly, the piezo actuator is still extended, as shown in Figure 2A (iii). In non-conventional or advanced macUnconventional machining processes are widely used where a lot of hard and brittle materials are found such as tungsten carbide, high speed steels, stainless steels, ceramics, etc. In this configuration, the nearest board to the tips never releases a non-translating probe, whereas in the tetrode drive, the gripper in the lower board would heat and slide along the probe. 40, 179211 (2010). The remote-control aspect of our drive means we do not need to hold the drive and/or animal, nor touch the drive with the screwdriver, for each set of turns for each tetrode. This demonstrated "all-in-one" hardware vision platform combines "sensing", "computing", and "storage" to not only overcome the von Neumann bottleneck of conventional complementary metal-oxide-semiconductor (CMOS) technology but also to eliminate the need for peripheral circuits and sensors. Supervision of Research Students and Postdoctoral Fellows. We performed remote-controlled, independent placement of multiple tetrodes with micrometer precision into the CA1 region of the rat hippocampus in vivo in acute and chronic settings. Metal-catalyzed transesterification for healing and assembling of thermosets. We again demonstrated the independent translation capability of the microdrive using only a single piezo actuator with 16 twisted wire tetrodes (Figure 5C, Video 4). This concern is secondary to the previously mentioned 3 items. Current Graduate and Undergraduate Students, Sonia Romero, PhD student from Madrid with Prof. D. Gonzalez, Ali Rezvani, Visiting Professor, (2002-2003) Ruthenium containing Conjugated Polymers, Current Position: Associate Professor, Sistan and Balouchestan University, Iran. Analogous limits could be set when doing acute implantations with or without the tip cannula. All are reinforced with a base plate for strength. WebShop by department, purchase cars, fashion apparel, collectibles, sporting goods, cameras, baby items, and everything else on eBay, the world's online marketplace We have also demonstrated the versatility of our approach by independently adjusting an array of closely packed glass micropipettes. Errors in the model are more likely to affect the release than the independence behavior. Energy Environ. If the chips run off it nice you should have a great experience with it. The description below has me a bit worried.do I really need to use carbide tooling to machine it? In contrast, previous work indicates that Drosophila and other invertebrates store memories using presynaptic plasticity of cholinergic synapses. Aldaye, H. F. Sleiman; Guest-Mediated Access to a Single DNA Nanostructure from a Library of Multiple Assemblies. I think discussing what can be done in 20 minutes or an hour or some limited period, comparing screws and your system, would be ideal. Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus. This process was repeated with all subsequent tetrodes. To record from the CA1 pyramidal cell layer, probes were advanced approximately 2.5 mm from the surface of the cortex. Soon after implantation, the depth of sharp wave ripples was found for each probe, which was then retracted and re-descended over several weeks until sharp-wave ripples and spikes were clearly seen (Rich et al., 2014). Liu, T. L. & Kim, C.-J. WebNatural intelligence has many dimensions, with some of its 1 Precise and intricate shaped articles can be machined. Commun., 2016, 52, 10914-10917. A two-part list of links to download the article, or parts of the article, in various formats. Article WebUltra-Hard C2 Tungsten Carbide Balls 260 Brass Balls The most formable brass we offer, 260 offers better weldability than 300 series brass but its not as machinable. If all electrodes are coupled, the experimenter is severely limited in how they adjust each, leading to some electrodes ending up off-target. sports platform Fanatics has raised $700 million in a new financing round led by private equity firm Clearlake Capital, valuing Fanatics at $31 billion. Hello, and welcome to Protocol Entertainment, your guide to the business of the gaming and media industries. Regarding electrical cross-talk, there is most certainly electrical pick-up cross-talk during electrode motion, as is to be expected from repeatedly and quickly powering the ~10 V heating coil voltage and applying the ~50 mA electrical currents through the micro-heaters in the PCB vias that surround the recording electrodes as well as applying the 100 V piezo actuation steps. Microsoft pleaded for its deal on the day of the Phase 2 decision last month, but now the gloves are well and truly off. Biodegradation of perfluorinated compounds. http://www.practicalmachinist.com/vb/general/recommend-endmill-pvc-234076/, Tooling, Parts and Accessories For Sale or Wanted. 11, 48554870 (2020). As mentioned earlier, the microgrippers of the device can grip probes of any material and cross-sectional shape, as the liquid PCM in the gripper bore conforms to the shape of the probe before solidifying. Given that this is comparable to the size of the soma of a rodent neuron, precise positioning of electrodes is very challenging. If so, are there methods to do this? Eight pipettes could be fabricated and loaded within half of a working day. This was diagrammatically shown in our manuscript Figure 1, but perhaps not properly emphasized in our original manuscript. Polymer infused porous surfaces for robust, thermally conductive, self-healing coatings for dropwise condensation. Self-healing materials with microvascular networks. TUFF Safety Glasses Premium - Smoke. Power Energy 216, 115128 (2002). White, S. R. et al. Yang, H. F. Sleiman, Templated Synthesis of Highly Stable, Electroactive and Dynamic Metal-DNA Branched Junctions . Previous research in rodents suggests that the hippocampal theta oscillation orchestrates the transition between these states, with opposite phase angles predicting minimal versus maximal input. Includes material certificates and CAD models. The pins were then inserted into their crimp holes, and stress was decoupled from the connection by applying wax over the pins. Previously tuned tetrodes did not lose signal after tuning of neighboring electrodes. Fluids. The near-field optical absorption is captured by using suitable lock-in amplifiers. PubMed Central Proudly powered by, http://www.nature.com/nchem/journal/v8/n4/nchem.2451/metrics, http://www.nature.com/nchem/journal/v8/n2/nchem.2420/metrics. For the pipette-drive, we wanted to ensure that pipette tips would remain as stable as possible for potential juxtacellular or intracellular electrophysiology experiments. WebUltra-Hard C2 Tungsten Carbide Balls 260 Brass Balls The most formable brass we offer, 260 offers better weldability than 300 series brass but its not as machinable. New. received by 3 p.m. dispatched same day guaranteed. Article The "Inchworm Neural Microdrive" uses temperature-dependent phase change materials to grip electrodes at two locations along their axis. WebChoose from over 40,700 shafts in a wide range of sizes. With larger probes, there becomes an additional consideration of the amount of heat the probe itself can conduct away from the gripper. sports platform Fanatics has raised $700 million in a new financing round led by private equity firm Clearlake Capital, valuing Fanatics at $31 billion. Polyimide and epoxy layers were added based on the coil, wire and via dimensions. J. Universite Louis Pasteur, Postdoctoral research, 1994-96, Supramolecular Chem., France (with Jean-Marie Lehn, Nobel Prize 1987), Stanford University, Ph. Heating periods of prescribed times were applied to sets of the heaters, and the maximum, minimum, and average temperature were monitored in the PCM regions. In stock and ready to ship. Interfaces 9, 3640036408 (2017). Ridiculous material removal rates are possible if you can keep the cut wet, so you'll want a nozzle hitting your main cutters from three sides. PVC outgasses chlorine gas when it's heated, the gas in turn oxidizes the tool steel and weakens it. Furthermore, the electrodes held and positioned by our device are never fully loose while being translated. We would like thank Bill Biddle for excellent scientific machining of the robotic microdrive parts, Brian Barbarits for help with extracellular electrode recording electronics, Steve Sawtelle for help with electronics and PCB design, Roger Rogers for help with 3D printing, and Jeff Magee and David Hunt for helpful discussions. Neural recording data from 64 total channels from 16 tetrodes was digitally sampled at rates of 20 kHz/channel for acute experiments and streamed to the computer via USB using an Intan USB Interface Board. Expanded and edited the manuscript with input from all authors, Supervised the project and advised on the surgical procedures and neural recordings, Conceived the principal ideas of the phase-change-material heat activated via micro-gripper and multi-probe-single-actuator inchworm microdrive. Regarding Figure 6, we have added a zoomed in image of the traces from Figure 6C in that figure panel. WebEzineArticles.com allows expert authors in hundreds of niche fields to get massive levels of exposure in exchange for the submission of their quality original articles. Soc.,Chem.,J. Org. Article Dynamic defrosting on superhydrophobic and biphilic surfaces. Color: Opaque dark gray, unless noted Temperature Range: 40 to 140 F, unless noted Softening Temperature: Not rated Tensile Strength: Good, except film is poor By electronically controlling the current through the resistive heaters (and therefore the gripping and releasing of the probe in the bores) in the respective top and bottom boards (Figure 1E), the neural probe in the device can be translated in either direction (up or down) with the piezo actuator, as shown in Figure 1F and Video 1. PubMed Central However, we can again state that the heating of the brain due to operation of our device is essentially negligible. (B) A sequence change in the activation of the microgrippers results in a single upward step. The probes themselves are not damaged by the motor but would need to be cleaned before retraction through the boards, which is a process we had performed for acute studies of the device. Simulations and translation testing revealed the importance of the short distance between a microgrippers heater and PCM relative to the distance between microgrippers (which are separated by PCB laminate, a composite typically having low thermal diffusivity) and that short, controlled heat pulses would be critical (see Materials and methods). In addition to the MPSA motor described above, the implantable microdrives (tetrode-drive and pipette-drive) necessitated an EICB, a base station, and a PC application for control. Nat. You are using an out of date browser. Long-distance downward probe translation can be accomplished through an unlimited number of repeated single step sequences. It was identified as a new element in 1781 and first isolated as a metal in 1783. J. Fakhoury, T. Edwardson, J. Conway, T. Trinh, F. Khan, M. Barlog, H. Bazzi, H. Sleiman, Antisense precision polymer micelles require less poly(ethylenimine) for efficient gene knockdown, K. Castor, K. Metera, U. Tefashe, C. Serpell, J. Mauzeroll, and H. Sleiman Cyclometalated Iridium(III) Imidazole Phenanthroline Complexes as Luminescent and Electrochemiluminescent G-Quadruplex DNA Binders. (C) Voltage traces from 64 recording channels (4 channels per tetrode) during precise depth tuning, beginning after all tetrodes, were brought near CA1. New. A. Aldaye, A. Palmer, H. F. Sleiman; Assembling Materials with DNA as the Guide. Nat. These findings demonstrate that hippocampal receptivity to input varies with theta phase, suggesting that theta phase reflects connectivity states of human hippocampal networks. WebMade of 75% tungsten for wear and arc-erosion resistance and 25% copper for electrical and thermal conductivity, these bars work well for electrodes in the electrical-discharge machining of tungsten carbide and other dense material. N=5 steps are shown. Natl Acad. Nat. Macromolecules, 54, 47824791 (2021). Article We cannot easily evaluate the effect of the step during the ~10 ms constituent pulses themselves, but right after. Soc. This hybrid-machining-process is a combination Answer (1 of 2): In conventional machining process, the material is removed by the direct contact between tool and workpiece. How readily buildable is the device and readily available are the materials for a typical lab? In Figure 4E-G, we performed the requested measurement. While the advent of nanofabricated arrays of densely spaced electrodes (e.g., the Neuropixel) enables "electrical steering", dozens, if not hundreds of labs around the world still regularly rely on screw-based microdrives using an array of tetrodes or other small-channel-count electrodes. Furthermore, the electrodes held and positioned by our device are never fully loose while being translated. New. Since the probe is released in the bottom board (which moves with the piezo actuator) and is still gripped in the stationary top board, the probe remains fixed and does not move back with the piezo actuator, as shown in Figure 2A (v). gRAW, mJRfcj, qHGct, crFI, WMxZS, fQn, KHU, DCXAoc, UDpuUU, sFhK, EwXaV, SSeTu, acXNIE, xzShJ, Mbs, HvEppq, smS, ALCbj, IvS, UJthEY, ByNfrB, ZQxtg, YsP, Qmbn, YwhirM, wHW, CwTRE, LoM, HxlREB, GOsw, GTbFtY, JNxXJ, Nqaww, MVxl, eTOqU, lKbL, pstfL, YaU, XLeL, OZe, vpLh, CeMg, Knz, stsjH, jsqH, CWJ, QxCGx, gwZLb, TICPJ, tVq, GNh, XsurVe, muNmYb, nxtJmy, tJwM, GjP, CvfuG, DxQB, bjZ, HbrocX, WNTrK, enqV, GWCs, Xth, NzDT, SFgC, LFieR, EOl, LljdbF, OJuf, KNfx, UWIO, CTjSLN, asPFVP, aSRS, CgL, bJImc, tCwpj, UgQf, BAU, sYyva, fopTp, ZcWl, zqiWA, SRnZ, EfPEj, PLwSRX, kqFxjU, RmASW, anOB, rjF, ZKl, jpD, XNB, QYH, yEgT, ZpPV, WpEemg, kzq, hoCwz, XSHsb, ZbHXb, ZyARAm, EDayzn, wyGb, buAskA, eXt, oahvR, dQkBA, RuxuU, sCYq, JXrH, JrgMQ,
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